Sentence examples for extract to detect from inspiring English sources

Serologic status to RhCMV was determined by enzyme-linked immunosorbent assay (ELISA) with an infected cell extract to detect RhCMV-specific immunoglobulin G (IgG) (28 ).

After 15 min of rotation, 100 μl of HeLa cell nuclear extract (to detect full-length TLS) or 3 μg of GST TLS-4 or -5 were added and incubated for 1 h at 4 °C with rotation.

This assay is based on intravenous injection of tumor cells into immunodeficient mice; 24 48 h later, the organs are extracted to detect the presence of human cells.

The two aliquots were used to perform an immunoblot with HeLa and Cos1 cell extracts to detect the endogenous JIP1 protein.

Viral DNA was extracted from each swab through a quick extract method to detect CMV DNA in saliva.

Multiple PCR reactions were performed directly on the extracted DNA to detect enterohemorrhagic E. coli (EHEC), enteropathogenic E. coli (EPEC), enteroinvasive E. coli (EIEC), enterotoxigenic E. coli (ETEC), and enteroaggregative E. coli (EAEC).

Indeed, these assays were performed only after two platelet washing cycles to ensure removal of extracellular NAC, and HPLC measures on treated platelet extracts failed to detect intra-platelet NAC (detection threshold ~2 μmol/l).

ten ul of DNA extract were used to detect and quantify viral genomes by real time PCR assays.

Nuclear extract were used to detect the expression levels of Jun D, Fra-1, NFκB subunit p65 and p50, and Lamin B. Twenty micrograms of whole cell lysate or 5 micrograms of nuclear extract was separated on a 10% SDS-PAGE gel and transferred onto a PVDF membrane.

Fehling's solution was added to the extract and heated to detect reducing sugar.

Laser treatment of the calendula extract is necessary to detect this biological activity.