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Ara h 1 was extracted and purified from defatted peanut flour as described previously by Marsh et al. [ 37] with the following modifications: after the extraction procedure, solid ammonium sulphate was added to the extract to a final concentration of 70%% saturation, and precipitation was carried out during 1 h in an ice bath.
Slow cooling and evaporation of the chloroform extract to a volume of 50 mL yielded good crystals of 3. The product was recrystallized two times from acetone.
The theoretical equations have been used in the prediction, with good precision, of the response of a polyculture in perfect mixing grown with beef extract to a step disturbance on the inlet concentration.
Many experiments can be required to determine how best to hybridize any given labeled extract to a particular array and how to block, wash, and postprocess (e.g., stain) the array so that the signal‐to‐noise ratio is maximized.
The flasks were supplemented with an additional carbon source, (glucose or sodium acetate), or additional nitrogen sources, NH4Cl, (NH4 2SO4, urea or yeast extract to a final concentration of 0.01% (w/v).
POD activity was assayed by adding 0.1 mL of the enzyme extract to a substrate mixture containing acetate buffer (0.1 mol L-1, pH 5.4), ortho-dianisidine (0.25% in ethyl alcohol) and 0.1 mL 0.8% H2O2 was added to 0.1 mL of the enzyme extract.
Similar(41)
Data elements of interest from each accepted study were extracted to a data extraction form.
The characteristics of the included studies were extracted to a piloted data extraction form.
It can also be extracted to a high chemical and isotopic purity from radioactive waste.
This data was then extracted to a separate channel containing three dimensional co-localized points only.
Clinical, epidemiological, radiology and laboratory data for each patient were extracted to a standardized clinical research form.
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