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In this study, our aim was to find a simplistic clinically viable method to extract the kidney function parameters from routine PET/MRI scans.
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So, the present work was designed to evaluate the antidiabetic effect of olive leaf extract in the kidney of diabetic pregnant mice and their fetuses.
Total RNA was extracted from the kidney and muscle employing the mirVanaTM RNA extraction kit (Ambion).
Total RNA was extracted from the kidney, lung, and heart with TRIzol reagent (Invitrogen, Carlsbad, CA, USA) according to the manufacturer's instructions.
Total RNA was extracted from the kidney using TRIzol reagent (Invitrogen).
For this purpose, total RNA extracted from the kidney, liver and mammary gland of Hpse-KO and wt mice, was analyzed using specific primers corresponding to Hpa2 (Table 2).
RNA was extracted from the kidney for microarray analysis.
Total RNA was extracted from the kidney using Trizol kit (Invitrogen, USA).
The integrity of total RNA extracted from the kidney was assessed using a Bioanalyzer 2100 (Agilent, Santa Clara, CA, USA).
Total RNA was extracted from the kidney and spleen of two healthy individuals using TRIzol reagent (Invitrogen).
Total RNA was extracted from the kidney using TriZol Total RNA Isolation Reagent according to standard procedures (Gibco BRL, Breda, the Netherlands).
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