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Finally, we compared our crude S30 extract system to the PURE translation system lacking RF1.
We directly compared our crude S30 extract system to the PURE translation system lacking RF1 in terms of the modified protein synthesis yield and the production cost.
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Our combined genetic and cytological approaches in Drosophila complement and extend existing work employing yeast genetics and Xenopusin vitro extract systems to characterize higher-order chromosome structure and function.
The resulting pQBI25-HL4-MIP or pQBI25-HL4-FLAG plasmid was in vitro-transcribed and translated in a TNT coupled wheat germ extract system (Promega) to produce GFP-fused peptides.
In this study, we present an information extraction system to extract related information on heart disease risk factors from unstructured clinical notes using a hybrid approach.
A nonlinear least-squares technique is then implemented to extract system parameters from laser vibrometry data of the diaphragm motion.
Recently proposed data analysis methods include, for example, multi-parameter estimation from hypersurface models [15, 16], artificial neural networks for classifying voltammetric signals by reaction mechanism [17], and bootstrap resampling to extract system parameters and their error distributions [18].
This extract system provides the ability to assess directly the roles of DNA replication, repair and transcription in driving expansions.
Leveraging modern bioinformatic analytics against intricate stem cell transcriptome readouts can extract systems biology features intractable to other approaches, and demonstrates a novel capacity for stem cells as advanced high resolution diagnostic tools (Fig. 6).
We then used a T nT Coupled Wheat Germ Extract System (Promega, USA) to perform in vitro transcription/translation reactions using SP6 control DNA and the modified vector.
We choose Xenopus egg extract as model system to study the effects of ATM on the PPP.
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