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The assay was based on the commercially available "E. coli S30 Extract System for Circular DNA" kit (Promega).
The mutant αHL monomers were first synthesized by coupled in vitro transcription and translation (IVTT) using the Escherichia coli T7 S30 Extract System for Circular DNA from Promega (Madison, WI).
Cell free translation assays— E. coli (E. coli S30 Extract System for Circular DNA, Promega) and rabbit reticulocyte whole cell extract (Rabbit Reticulocyte Lysate System, Nuclease Treated, Promega)—were performed as recommended by manufacturer.
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These include a protocol for the preparation of nucleoplasmic extract (NPE), which is a soluble extract system useful for studying nuclear events such as DNA replication and checkpoints.
Both reviewers make the point that the first part of the paper (extract systems and the implications for changes in the spindle throughout development) is profound and very important, while the data presented in the second part (kif2a and importin α are responsible for the difference between spindle length) are not strong enough to provide extensive, mechanistic insights.
In this section, we introduce a method for extracting system matrices of a class of parametric problems, so that the parametric representation of the models in state-space form can be derived.
Over the past decade, several groups have demonstrated the power of using crude extract CFPS systems for site-specific incorporation of NSAAs.
The fast extraction system is used for extracting short bunched beam horizontally in single-turn.
The aim of the present study was to prepare and characterize polymeric nanoparticles loaded with Brazilian red propolis extract and evaluate the cytotoxic activity of "multiple-constituent extract in co-delivery system" for antileishmanial therapies.
More importantly, IP geolocation can play a significant role in network security, such as tracing cyber fraud and attacks and extracting the system logs for computer forensics.
The development of "multi-compound extract in co-delivery system" for target tissues arises due to the high prevalence of the number of cases of cancers [55] and the increase in drug-resistance during leishmaniasis treatment worldwide [56 59].
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