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The appearance of a brown color in AgNO3-treated leaf extract suggested the formation of AgNPs (Gurunathan et al. [4, 16]; Sathiya and Akilandeswari [26]).
Similar levels of apo LasRLBD relative to the rest of proteins in the soluble fraction and in the total protein extract, suggested that the polypeptide was highly soluble.
The LDH release curves for MCF-7 and MDA-MB-231 cell lines treated with different concentrations of the kernel extract suggested that the cytotoxic effect of the extract was concentration-dependent.
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Moreover, neutrophils from cattle and mice form NETs in response to OO extract, suggesting possible common mechanisms in NET formation shared by mammalian species in responding to nematodes.
The capacity of bovine neutrophils to release significant levels of NETs in response to low concentrations of OO extract suggests that this response may be viable in vivo, where the actual abundance of parasite antigen can be quite low.
Inhibition in inducible nitric oxide synthase (inos) gene expression in the cells treated with the plant extract suggests an inhibition at the transcription level.
This modest activity disappeared in the double knockout extract, suggesting that it was due to pol λ.
The absence of a clear full-length FLG2 band in the SC extract suggests that FLG2 is processed within the stratum granulosum.
This, together with the fact that SR45 complements S100 cell extract, suggests that it is involved in both constitutive and regulated splicing.
Histone H1 was exclusively detected in the nuclear fraction, whereas MEK1 was totally expressed in the cytoplasmic extract, suggesting that we successfully fractionated cell lysates (Fig. S1 [C]).
We showed that SR45 was able to splice a pre-mRNA substrate in a splicing-deficient extract, suggesting that it is a splicing factor (Figure 1B).
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