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We have here adopted the same approach of extract screening to look for antigens that might form the basis of a serological test for T. b. rhodesiense infection, but have followed up with further tests using recombinant proteins.
Specific fields of interest for these technologies are metabolic profiling of bioactive metabolites, natural extract screening, and the screening of libraries for bioactives, such as parallel synthesis libraries and small combichem libraries.
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We extracted screening results from a nationwide clinical diabetes database to calculate annual referral rates to ophthalmic clinics.
Among all the plant extracts screened here, two extracts, AK and AT, showed particularly potent activity with IC50 values of 13.73 μg/ml and 27.00 μg/ml, respectively, against HeLa cells.
Additionally, 1 μl of extracted, screened and quantified gDNA of three porcine (L1, L17 L71) C. pecorum samples were run on a 1 % TBE agarose gel to confirm high molecular weight DNA prior to whole genome sequencing.
Of the seven mangrove extracts screened aqueous extract from Ceriops tagal imparted total protection to shrimp from WSSV when challenged by both methods.
Of a total of 7500 plant extracts screened on 60 human cell lines, the most active were further tested on MCF7, renal cancer and melanoma cell lines.
Under this research programme seven mangrove plants were collected, identified and aqueous extracts screened for their protective effect on the giant tiger shrimp Penaeus monodon against WSSV.
The team's candidate is derived from a fungus called Streptomyces platensis that was recovered from a South African soil sample--one of 83,000 natural extracts screened for antibiotic activity.
Of these 126 extracts screened, 21 extracts tested positive for PPA inhibition (Table 2).
Among plants extracts screened on multiple cell lines, four species showed a degree of selectivity.
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