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The objective of this study was to evaluate the influence of the addition of 0.00 5.00 g/100 g microencapsulated omega-3 fatty acids (MO) and of 0.000 0.100 g/100 g rosemary extract (RE) on the technological and sensory quality of white pan bread, following a 22 central composite rotational design (CCRD).
The total phenol and flavonoid contents of in vitro shoot extract (ISE), in vitro root extract (IRE), callus extract (CE), in vivo shoot extract (SE), and in vivo root extract (RE) were shown in Figure 2.
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Fig. 2 Computational steps workflow for extracting re-computable molecular structures from PDF articles as implemented in ChemEngine.
Any samples for which mtDNA and nDNA disagreed were re-extracted, re-sequenced and/or cloned and sequenced.
Lipids were extracted, re-dissolved in toluene, and separated with TLC prior to quantification as previously described [56].
When observing some phylogenetic inconsistency, sequencing electropherograms were rechecked; DNA sample was re-extracted or re-sequenced when persisting some doubt regarding the variant in question.
If the detected concentration was out of the linear range, samples were appropriately diluted with PBS, re-extracted and re-analyzed.
To eliminate the possibility that ambiguous sites were caused by sequencing errors, Morris-Pocock et al. [ 14] re-extracted and re-sequenced 20% of brown and red-footed booby individuals that had ambiguous sites: all sequences (including ambiguous sites) were identical.
Additionally, a subset of individuals with sequence ambiguities in the current study were re-extracted and re-sequenced at the Queen's Ecology, Evolution and Behaviour Core Genotyping Facility, as above.
The extract was re-suspended in H2O and partitioned with n-hexane, ethyl acetate, and n-butanol sequentially.
The dried extract was re-constituted in normal saline (0.9 % NaCl).
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