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A series of PCR-based analyses was then used to assess DNA extract quality and effect on microbial community profiles.
For each of the three platforms, the ten subsample DNA extracts were pooled to generate DNA stocks for subsequent analyses to assess DNA extract quality and its effect on the microbial community structure, while minimizing the effect of small sample volumes.
This was done in order to assure extract quality and consistency from each and every extract.
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The clinical implications of allergen polymorphism in the olive pollen are examined in detail in [ 51], in particularly how cultivar differences may affect extract quality, diagnostic and therapeutic efficacy and safety, and the development of new vaccines.
These characteristics have made it challenging to extract quality RNA from these and other grapevine tissues [ 21, 22].
Total RNA was extracted, quality controlled and stored as described above.
Papers were screened, data extracted, quality appraised and analysed by two individuals, with a third party for disagreements.
Data were extracted, quality assessed and systematically mapped according to key findings through generation of an iterative framework.
First, whole saliva samples with volumes of at least 100 μl were sufficient to extract good quality and quantity DNA.
Whole saliva samples with volumes of at least.10 ml were sufficient to extract good quality and quantity DNA.
After total RNA of different organs were extracted, the quality and concentration of different total extracted RNA were evaluated by 1% agarose gel electrophoresis and spectrophotometric analysis, respectively.
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