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Cells were grown for 48 hours and then harvested for cell extract preparation.
This might influence the degree of sensitization or the outcome of treatment strategies in dependence of the applied extract preparation.
Top flowering parts were used for extract preparation (80% flowers and 20% rest of the plant).
Prior to extract preparation, the plant leaves were cleaned thoroughly using deionized water and then cut into small pieces.
Five organic solvents, i.e., chloroform, hexane, ethyl acetate, butanol and methanol were used for the extract preparation.
The toxicity of the extracts was assessed after extract preparation.
Cells were then collected or fixed after hydroxyurea release for cell extract preparation or immunohistochemistry analysis.
The protocol for cytoplasmic and nuclear extract preparation was adapted from previously published procedures [38], [39].
Cells at ∼80 90% confluence were generally used for extract preparation.
All maturase extract preparation steps were carried out under aerobic conditions.
Nuclear extract preparation from cells was carried out as previously described [17].
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