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This paper presents a capillary-driven microfluidic device that uses surfactant-added poly(dimethylsiloxane) (PDMS) to extract plasma from the blood without any external driving force.
Cyclodextrins have long been used to extract plasma membrane cholesterol, which can result in depletion of up to half of the total cell cholesterol content within one hour of treatment [9].
In most reported studies on lipid rafts, a detergent resistance-based biochemical technique is used to extract plasma membrane in the nonionic detergent Triton X-100 at 4°C, and thus to sort soluble and insoluble components via sucrose density gradient centrifugation.
The blood samples were immediately processed to extract plasma by centrifugation and stored at −70 °C until analysis.
To extract plasma and serum the tubes were, after appropriate treatment, centrifuged at 1500 g for 10 min at 4°C.
Donors received money to donate blood; In order to increase donation frequency, in most collection centers blood was pooled in machines to extract plasma.
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After extraction, SPE extracted plasma with cathinone and mephedrone samples were either analyzed directly by LC-MS/MS or first spiked with known concentrations (0 250 ng/ml) of cathinone and mephedrone as "standard addition" samples.
The determination of the extraction recoveries was performed by comparing the observed peak areas of bergapten in extracted plasma samples with those of the bergapten in non-processed plasma samples at the same theoretical concentrations.
Inside, for about an hour, Ms. Delgado lies hooked to a machine that extracts plasma, the liquid part of the blood, from a vein in her arm.
In 1996, Dr. Gao Yaojie discovered that many rural residents of Henan Province had become infected by selling blood to government-sanctioned collecting stations that then pooled the blood, extracted plasma and reinjected the donors.
The buyers pooled the blood of many people, extracted plasma to sell and then re-injected the remaining pooled cells back into the donors -- perhaps the most efficient method ever devised to spread blood-borne diseases like AIDS and hepatitis.
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