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Methanol has been intensively used to extract plant phenols, and its extraction efficiency was generally higher than ethanol (Kumaron and Karunakaran 2007).
The main advantage of MAE over ultrasonic assisted extraction and Soxhlet extraction is that, it can be used to extract plant metabolites at a shorter time interval (Afoakwah et al. 2012).
Raw pine cone (RPC) was treated with toluene ethanol mixture to extract plant components and modified with hexacyanoferrate complex (HPC).
In all these studies, the AgNPs were removed from extract (plant extract) after the synthesis and their anticancer activities were studied.
Considering components of the extract, plant sterols, in particular β-sitosterol, were shown to influence skin cell migration and proliferation.
Numerous commercially available kits are used to extract plant DNA in high quality from leaves; these methods will not be reviewed here.
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The chosen solvent was methanol (M3641, Sigma-Aldrich, Gillingham), which has been proven effective for extracting plant materials [69].
Genomic DNA (gDNA) was extracted plant tissues using the DNeasy Plant minikit (Qiagen, the Netherlands).
In general, the particles appear more aggregated in the two extracted plants, with a larger size for FeNP of ginger.
The formula used was FIC ampicillin = MIC of ampicillin in combination/MIC ampicillin alone, FIC plant extract = MIC of plant extract in combination/MIC of plant extract alone, and FIC index = FIC ampicillin + FIC plant extract.
This solution was treated as a source extract (containing plant extract) and was utilized for subsequent operations.
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