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Each extract of transcribed data was subjected to thematic analysis [ 37].
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A water extract of Artemisia capillaris (AC) have been transcribed for liver protection in traditional Korean medicine [ 10].
Twenty five μg of each total RNA extract was reversely transcribed using random hexamers, 40U RNaseOUT™ and 400U Superscript III RT (Invitrogen) at 42 °C for two hours.
One μg of extracted RNA was transcribed into complementary DNA (cDNA) according to a standard protocol using Omniscript Reverse Transcription Kit (Qiagen, Hilden, Germany).
RNA from the resistant strain and a standard laboratory susceptible strain, of both sexes was extracted, reverse transcribed and labelled with either Cy3- or Cy5-dye.
After 8 or 24 hr of exposure, RNA was extracted, reverse transcribed, and amplified by polymerase chain reaction with degenerate primers to visualize alterations in gene expression.
Briefly, we estimated the mitochondrial purification yield from the median log2 values of the mitochondrial fraction/total cell extract ratios of 33 RNAs transcribed from the mitochondrial genome (8 mRNAs, 2 rRNAs and 23 tRNAs).
The aim of the protocol was to maximize the number of plasma HIV RNA molecules that were extracted, reverse transcribed, PCR amplified and finally subjected to UDPS.
Cellular RNA was extracted and transcribed to cDNA.
In a next step, we carried out cysteine protease activity measurements with nodule extracts to determine potency of transcribed cystatins.
Briefly, 0.5 μg of total RNA extracted was reversely transcribed by iScript cDNA synthesis kit (Bio-Rad, Hercules, CA).
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