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Secondly, the strain was exposed to trace metals, in a liquid malt extract medium.
In addition, use of a nutrient medium including CSL and urea did not have such an adverse effect on initial set and compressive strength as compared to a urea and yeast extract medium.
The maximum emulsification index was obtained under the optimal condition of 29.31 g L−1 mannitol; 2.06 g L−1 yeast extract, medium pH 6.97 and 5.69 g L−1 NaCl.
The reaction of chalcones 3 a-g) with phenylhydrazine hydrochlorides 4(a-b) in acetic acid (3 a-gmedium and also with freshly phenylhydrazine extract medium under reflux conditions produced thydrochloridesppended pyrazoles 5(a-l) in moderate yields.
Twenty pH-controlled batch cultures with Lactobacillus helveticus were carried out in whey permeate-yeast extract medium according to a composite design with three factors: pH setpoint, and yeast extract and initial whey permeate concentrations.
a Minimal glucose medium; b yeast extract medium.
The first phase was a preselection study employing malt extract medium without agitation.
The liquid culture used in the experiments was prepared from stock culture using sterilized malt extract medium.
40643_2017_180_MOESM2_ESM.xlsx Additional file 2. Gene essentiality analysis in minimal growth medium and yeast extract medium by flux balance analysis.
Transformants expressing mnp1 under the control of actin regulatory sequences produced MnP activity in malt extract medium, while the parental strain A15 did not.
In addition, the essentialities of individual gene of Y. lipolytica grown in both minimal growth medium and yeast extract medium were investigated based on the four above models.
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