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It is further shown that multicellular transmigration of the BM is less susceptible to pharmacological MMP inhibition than multicellular invasion in composite collagen/basement membrane extract matrices.
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This peak intensity was averaged over 50 80 scans (approximately 60 s) and compared to standards made up in extract matrix.
The limit of quantification was found to be 500 ng/mL Elodea extract matrix ±12% mean standard deviation.
Fig. 3 Spiking experiment and identification of catechin and EGCG in tea extract matrix using phenyl-hexyl core-shell column.
Identity of each antioxidant in the tea extract matrix was confirmed by a set of spiking experiment.
Spiking experiments were also conducted for proper identification of peaks within the tea extract matrix as well as the basis for the measurement of trueness (Fig. 3).
hPSCs are typically grown on biological substrates such as human or mouse feeder cells, extracted matrices (e.g. Matrigel) or recombinant proteins (e.g. laminin, collagen, fibronectin and vitronectin), all of which are expensive, variable and/or labile [ 8].
After removing fibroblasts, extracted matrices were re-cultured with breast epithelial cells of assorted characteristics: MCF-10A (non-tumorigenic), MCF-7 (tumorigenic, non-invasive), and MDA-MB-231 (tumorigenic, invasive).
Peptide mass fingerprinting confirmed the presence of fibrillin in the extracted matrix.
Rotary shadowing electron microscopy showed that the extracted matrix had some fibers that resembled fibrillin microfibrils.
The insoluble extracted matrix preserved fibrillar structures and a major portion of the extracted proteins migrated as FN monomers on an SDS gel under reducing conditions.
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