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However, the high complexity of the antennal extract mixtures and the low concentration of single proteins in the extract limit the application of top-down experiments.
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A two-stage cultivation strategy was designed in order to create a yeast extract limited environment so that plasmid-free cells could not grow and overtake the recombinant cells.
These plant extracts limited the depletion of intracellular glutathione content caused by rotenone exposure and demonstrated potent anti-apoptotic effects, warranting further investigations into their neuroprotective potential.
Procedure solvent blank samples representing every seventh sample were analyzed in the same way as the serum extracts Limits of quantification (LO Qs were defined in direct relation to the amount of interference of PBDEs in the blank samples.
Extract limits preferred.
Later, analytical approaches are followed to extract global limits and compare with those from the literature.
Reiner, with his notorious microscopic baton beat, needed no more than a glance and a blink of an eye to extract the limits of musical dynamics and articulation from the orchestra.
To do so, the incubation with the RAG1/2 extract was limited to 5 min, and the two 38 and 27 nucleotides (nt) fragments corresponding to respectively RAG1/2-mediated 12- and 23RSS nicks, were monitored (Figure 3).
However, J. regia extract had limited effect on the resistance of E. coli strain to aztreonam.
The EMSA assays employ HeLa nuclear extract, thereby limiting our findings to the TF expression repertoire of only HeLa nuclei.
Note that the concentration of the extract was limited at 500 μg/ml to avoid toxicity on cell viability that may be caused by the extract itself.
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