6 μL of NBD-PS or NBD-PC, dried from a concentrated chloroform stock and resuspended in HEPES buffer to 30 μM, was then added, and 50 μL samples were taken at indicated time intervals and diluted into 200 μL of HEPES buffer (pre-cooled on ice) with 1% fatty acid-free BSA, to extract label remaining in the outer leaflet of the membrane, or without BSA, to measure total fluorescence.
To extract labeled proteins, 1×109 bacteria were resuspended in 500 µl of 50 mM Tris-HCl (pH 8.0), 100 mM NaCl, 2 mM EDTA, 0.2% SDS and boiled for 5 min. Biotinylated proteins were then affinity-captured with EZview Red Streptavidin Affinity gel (Sigma-Aldrich) according to manufacturer's instructions.
Then, the strips were cut into 44 equal pieces along pH gradient, and each piece was consecutively incubated in 100ul of 0.1% FA, 0.1%FAcetonitrileitrile (ACN), and 0.1%ACN80%ACN for 10 min each to extract labeled peptides from gel.
From an AGROVOC URI there is a possibility to extract labels of a concept in all the languages available in AGROVOC: those labels can be considered as "translations" of a query term, so that they can be used to expand the user's query to include the translation of terms in different languages.
For our experiments, these extracted labels also serve as the ground truth.
The extracted labels may correspond to the speakers in the speech corpus if the data is relatively clean.
RNA was extracted, labeled and hybridized to Affymetrix Mouse Genome 430 2.0 Arrays using standard protocol (Medvedovic et al., 2009).
miRNAs were then extracted, labeled and detected by microarray.
The RNA extracts labeled with Alexa 594 were used individually as labeled tissue samples.
MicroRNAs from aliquots of 2 serum samples from cancer patients (1 prostate and 1 colon) were extracted, labeled and hybridized to arrays, and after one freeze/thaw event, new aliquots were again extracted, labeled and hybridized to a second array.
Table 3 and Table 4 state the description of the extracted labels and items respectively with the discriminant coefficients for the DA of 22q11DS+ASD versus heterogeneous ASD.
