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After filtration, the filtrate was concentrated by evaporation at 70°C under reduced pressure on a rotary evaporator to afford 45 g of the methanol extract, corresponding to an extraction yield of 5.6%.
A total of 300 g of the powder of Guibourtia tessmannii were macerated in 1.5 L of methanol for 72 hours to yield, after solvent evaporation under reduced pressure, 34.1 g of brownish extract corresponding to an extraction yield of 11.37% (w/w based on the dried starting weight).
A total of 300 g of the powder of Mondia whitei roots were macerated in 1 L of hexane for 72 hours to yield, after solvent evaporation under reduced pressure, 1.145 g of brownish extract corresponding to an extraction yield of 0.38% (w/w based on the dried starting weight).
In order to obtain the ethanolic extract, ground root bark (1 kg) of Dracaena arborea was macerated with ethanol (95%) (5 l, 2x) for 72 hours to yield, after solvent evaporation under reduced pressure, 30 g of a brownish extract corresponding to an extraction yield of 3%.
Ground leaves (600 g) of Bersama engleriana were mace-rated with methanol (3 L; 2 × ) for 72 h to yield, after solvent evaporation under reduced pressure, 16.5 g of brownish extract corresponding to an extraction yield of 14.29% (w/w based on the dried starting weight).
The decoction was filtered through a Whatman filter paper no.1 and evaporated to dryness in an air oven at 40 °C to give 19.72 g of aqueous extract corresponding with an extraction yield of 3.25 %. 200 g of the stem bark powder was soaked with 1.5 l of methanol for 72 h.
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For all the genes we extracted corresponding orthologs in mouse, rat, chicken, fugu and danio genomes.
Protein extracts corresponding to 300 MII oocytes or one-cell embryos were applied to an Immobiline drystrip (pH 3 10, GE Healthcare) and subjected to isoelectric focusing with a multiphor apparatus (GE Healthcare) as the first protein separation.
A list of proteins was extracted corresponding to the peptides selected above.
Items which did not conceptually fit in their extracted corresponding factors were also discussed at this stage.
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