Exact(9)
The effects of AgNPs were compared with the positive antibiotic (Nalidixic acid 10 µg/mL) and negative (supernatant extract) controls.
To eliminate the absorbance produced by plant extract, appropriate extract controls without the enzyme were also included.
To eliminate the absorbance produced by plant extract, appropriate extract controls with the extract in the reaction mixture except for the enzyme were also included.
The controls consisted of untreated infected cells for virus control, confluent monolayer cells were infected with the virus at 10 TCID50 ml−1 of virus; treated uninfected cells for plant extract controls; and untreated uninfected cells for cell control.
The mean value for the negative control (BSA or acetic acid), or preferably individual extract controls, should be subtracted from the mean values of the unknowns to eliminate background noise for the most accurate final concentration.
Extracts of the samples and extract controls were subjected to PCR with standard avian mtDNA primer sets (Cytochrome b, ND2; [ 49]) followed by sequencing of positive products to confirm the isolation of turkey DNA from the toe pads.
Similar(51)
Glutathione and yeast extract control and brighten pigment.
Group E rabbits: were neither given salt loaded diet nor treated with the extract (Control).
The cells grown in absence of the Ag NPs were taken as the microbial control and the cells grown only in extract were taken as the extract control.
Biomass yields with 5 mM oxalate and 5 mM formate were generally consistent with the 0.1% yeast extract control though higher concentrations were toxic.
An extract control sample from unexposed filters was processed identically.
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