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The use of Triton X-100 in this procedure, and potential nuclei extract contamination does not appear to be detrimental to subsequent reactions based on the following observations: 1) 0.1% nuclei are repeatedly washed after Triton extraction (see above).
Phenol chloroform (1 1) was used to extract contamination, and the genomic DNA was precipitated with ethanol (90%).
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It yields not more than maximum 6% of the weight of the date, the quality of the syrup per se is quite good, because it is the direct natural fruit extract, but contamination with foreign matter, because of the rudimentary ways the product is collected, is quite high.
To remove any residual cytoplasmic extracts contamination, cells were washed using buffer A, followed by PMSF added PBS.
Lerner assesses the diverse strategies used by affected communities to win legal settlements, educate and mobilize their residents, block permits and expansion of polluting facilities, force cleanup of contamination, extract concessions and pollution reduction from fenceline industrial plants, and target enforcement by government.
Therefore, we can extract the phase contamination function from the contaminated sea clutter to get the decontamination function.
GC analysis of the hydrocarbon fraction of the core extract indicated that contamination may be diesel derived.
Finally, the selected spots are automatically extracted under contamination-free conditions and then transported into a standard microtiter plate.
Krings used a number of careful--and expensive--laboratory techniques to guard against contamination and extract his sample.
Since most commonly used nucleic acid quantification procedures evaluate both RNA and DNA simultaneously, we explicitly tested each RNA extract for potential DNA contamination by means of PCR amplifications.
Two common forms of control exist: a non-specific antibody such as IgG to control the immunoprecipitation, and sequencing of whole cell extract to control for contamination and other possible sources of error.
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CEO of Professional Science Editing for Scientists @ prosciediting.com