Sentence examples for extract cells were from inspiring English sources

To assess cytotoxic activity of the extract, cells were exposed to EEP over 72 h.

Following a 12 h incubation with American ginseng extract, cells were washed and then IFN-γ (100 U/ml media) was added to activate the macrophages.

For preparation of CE/NE extract cells were harvested as described above and then first re-suspended in harvest buffer containing 10 mM HEPES (pH 7.9), 50 mM NaCl, 0.5 M Sucrose, 0.1 mM EDTA, 0.5% Triton-X 100 and protease inhibitor cocktail (Calbiochem, USA).

To study the interaction between DOX and B. serrata extract, Cells were treated with various dilutions of DOX in the presence or absence of B. serrata extract at 10, 20, and 30  μg/mL and IC 50 values were determined by plotting the percentage of cell survival as a function of drug concentration [ 18, 19].

To prepare the whole-cell extract, cells were washed with PBS once and harvested by scraping them in 1 ml lyses buffer (50 mM Tris-HCl pH7.4, 150 mM NaCl, 0.2 mM EDTA, 0.2% NP-40NP-40, 10cerin, 1M β-Me, 1 μg/ml Aprotin, 0.5 μg/ml Leupetin, 0.1 mM Na3VO4, 0.5 mM 4NPP, 0.5 mM NaF, and protease inhibitors).

After 3 min of extractions extracted cells were immediately cooled down on dry ice.

To prepare whole cell extracts, cells were rinsed with phosphate-buffered saline (PBS), adjusted with NaCl to the same osmolality as the medium, then lysed with RIPA buffer.

Moreover, extracted cells were viable and could be expanded in culture to produce BFU and CFU.

Directly extracted cells were seeded onto 10-cm2 flaskettes (SLS) at a cell density of 0.2 × 106 cells per flaskette.

For whole cell extracts, cells were collected at 400 × g for 3 min and washed with PBS.

To prepare total cell extracts, cells were harvested, washed in PBS, and lysed with RIPA buffer.