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Data were processed to extract cells that were tracked unambiguously for >10 consecutive frames.
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We tested whether our cell isolation method could extract larval cells that are present in vivo at a substantially lower density than muscle and neural cells types.
As mentioned above, SILAC labeling is only applicable when using extract from cells that can be grown in culture.
Attempts to measure a combined Bcd and EtfAB activity were not successful using crude extract from cells that expressed both bcd and etfAB.
From this he extracts precursor cells that can go on to form the muscle on the outside of the bladder and the specialised cells within it.
Total RNA was extracted from cells that were harvested at 20 and 40 h.
The dropping-RT-PCR was carried out on RNAs extracted from cells that were synchronized by double thymidine block at T0 (G1/S) and T+8 (G2/M).
The bacterial chromosomal DNAs were extracted from cells that had been cultured overnight by using a genomic DNA preparation kit (Tiangen, China) with lysostaphin at 10 mg/ml and RNaseA at 25 mg/ml for the lysis step.
Two samples were run on each TLDA card; RNA extracted from cells that were hsa-pre-miR-183 transfected and cells that were pre-miR-negative control transfected.
Total RNA extracted from cells that had been transfected with sense 5′-end RNA-1 was run for RT using a primer (850-877R: 5′-TCAGTTTTCATGAGATATCGTGTGTGGC-3′) complementhey to the sequence of nt 850~877.
Whole cell extract of Neuro2A cells (that express both P53 and HIPPI endogenously) showed positive co-immunoprecipitation of HIPPI with P53.
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