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However, the figure shows that at concentrations between 15 and 30 ppm of the GBR extract, cell viability reduced drastically compared to BR.
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Adjusting to the desired 5 80 μg/mL concentrations of extracts, cell viability determination and fitting of response curves, and the harvest of treated cells followed previously described methods [ 5].
The influence of the extract on cell viability was also studied using the MTT method.
Since oxidative stress and inflammation are also related to diabetes impairments, the effect of the extract on cell viability after UV radiation injury and the inhibition of COX-1 enzyme were also evaluated.
The impact of the galangal rhizomes extract on cell viability was quantitated by the MTT assay.
At 125 μg·mL−1 and 250 μg·mL−1 of plant extract, the cell viability decreased with time.
Results demonstrated that the extract decreased cell viability in a concentration-dependent manner.
The effect of the extract on cell viability and gene expression levels described below were assessed to standardize the method of extraction.
In accordance with its moderate antioxidant activity found in this study, pretreatment of D. reticulata extract increased cell viability up to only about 78%.
MTT assays were performed with different concentrations of CLE (GAE) in both the cell lines at the 12 h and 24 h time points to assess the effect of the extract on cell viability.
The aim of this work was to evaluate the in vitro antitumor activity of Portuguese propolis on the human colon carcinoma cell line HCT-15, assessing the effect of different fractions (hexane, chloroform and ethanol residual) of a propolis ethanol extract on cell viability, proliferation, metabolism and death.
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