Exact(17)
Kyrklund T. Two procedures to remove polar contaminants from a crude brain lipid extract by using prepacked reversed-phase columns.
We developed a purification method from an ethanolic extract by using a diatomite-based purification protocol.
EC and pH were measured in 1 2 (w:v) aqueous extract by using a pH/Ion meter (Metller Toledo. EL20-Educational line pH, EL3-Educational line conductivity).
The analysis revealed that five peptide sequences have similarity with the stretches of secreted lipoprotein of Methylomicrobium album BG8 (Protein View: gi|381152247) and displayed 70% coverage with a significant homology (Figure 5). Figure 3 Separation of the antimicrobial lipopeptide from acidic methanol extract by using reversed-phase HPLC.
(B ) IB showing efficient depletion of endogenous ORCA from U2OS nuclear extract by using ORCA antibody.
Hemoglobin quantification was also performed on the total embryo extract by using a modified cyanomethemoglobin method [ 28].
Similar(43)
Histones were extracted by using the acid extraction method63.
Data sets were extracted by using the feature extraction software (v9.5.3.1/Agilent Technologies) using a standard protocol.
Data was extracted by using a standardized data extraction form.
Total RNA was extracted by using the Omega RNA extraction kit (Omegabiotek).
DNA was extracted by using Qiagen (Crawley, West Sussex, United Kingdom) DNA extraction kits (16 ).
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