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Each sample was re-extracted and run in duplicate.
From each sample, DNA was extracted and run on the real-time PCR assay.
Total cellular DNA was extracted and run on CsCl-bisbenzimide (1.67 g/ml CsCl, 200 μg/ml bisbenzimide) isopycnic gradients as described [ 59].
To analyze whether the matched sequence could form a suitable hairpin, sequences surrounding the matched sequence (100~200 nt to either side) were extracted and run through RNAfold.
Thus, to further confirm the identity of the detected S100A4 variants, regions of interest were excised from gels and in-gel tryptic digestion performed, upon which peptides were extracted and run on MALDI-TOF.
For each target plate, four replicate samples of a quality control human serum (Sigma-Aldrich, Dorset, UK; #S7023) were extracted and run alongside case control samples to monitor inter-assay variation.
Real-time qPCR methodologies were developed adequately, were rapid and specific as it can be seen in Additional file 2: Figures S2 and Additional file 3: Figure S3 when the real-time PCR products from positive cDNA samples were extracted and run on a 2%% w/v agarose gel: variants A and E produced the expected bands at sizes of 439 and 259 bp.
Equal volumes of each extract and medium were run on a 10% SDS polyacrylamide gel (Biorad), proteins were transferred to nitrocellulose and Western blotting performed with rabbit anti-GFP primary antibody (1∶1000) and IRdye800-congugated anti-rabbit secondary antibodies (Rockland, 1∶3000).
Extract it and run it.
(Urban) The majority of the women interviewed spoke of their satisfaction with supplements such as glucosamine, fish oil and vitamins, although a number of the rural women commented on the difficulty of obtaining some products, for example, "There's only one chemist in town who stocks olive extract and if he runs out I sometimes have to wait for weeks for a new lot to come in".
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