It is invaginated and forms an extracellular room that is connected to the reservoir by the canal.
Bi = basal infoldings, C1 and C2 = canal cells, Cc = cuticular canal, Mv = microvilli, Nc1, Nc2 = nucleus of canal cells, Nep = nuclei of epithelium cells, Re = extracellular room, V = vacuole, Blue, nuclear staining; Red, Bodipy-stained intracellular membrane; Magenta, false color-coded autofluorescence.
Slices were allowed to recover for 60 minutes in oxygenated extracellular solution at room temperature.
Oocytes were continually perfused with extracellular solution at room temperature containing: 98 mM Na-methanesulfonate (MES), 2 mM K-MES, 2 mM CaCl2, 10 mM HEPES, pH 7.0.
For the recordings, the glass coverslips were transferred into a recording chamber where they were continuously perfused (2 3 ml/min) with the extracellular solution at room temperature.
After blocking in 10% goat serum for 10 min at room temperature, extracellular bacteria were stained by a Mycobacterium-specific rabbit polyclonal antibody (Cygnus Technologies, Southport, NC, USA).
Room-temperature extracellular biosynthesis of gold nanoparticles (Au NPs) was achieved using Escherichia coli K12 cells without the addition of growth media, pH adjustments or inclusion of electron donors/stabilizing agents.
Extracellular recordings were performed at room temperature in apical dendritic layers of CA1 area using glass micropipettes filled with 2 M NaCl with a resistance of 2 6 MΩ.
Dispersed mouse pancreatic acinar cells were pre-incubated at room temperature with extracellular solution containing 10 μM GSK-7975A or 10 μM PD150606 or 0.1% DMSO for 30 min.
All measurements were performed at room temperature in extracellular haemolymph-like solution (HL-3; Stewart et al., 1994) containing (in mM): NaCl, 70; KCl, 5; MgCl20 20; NHCO3, 10; trehalose, 5; sucrose, 115; HEPES, 5; CaCl2, 1; pH adjusted to 7.2.
Miniature excitatory postsynaptic current (mEPSCs) were recorded at room temperature in extracellular solution (155 mmol/L NaCl, 3 mmol/L KCl, 3 mmol/L CaCl, 10 mmol/L HEPES, 10 μmol/L bicuculline methiodide, 500 nmol/L tetrodotoxin, pH 7.35, 310 320 mOsm).
