(i k) Extracellular localization of the tested complement proteins.
For detection of extracellular localization of sHA1, cells were removed from ECM prior fixation step by incubation in 25 mM NH4OH for 20 min at room temperature keeping the endogenous ECM intact.
While we have not compared the enzyme activity or secretion of the R565Q mutant and wild type proteins, our data suggests an altered extracellular localization as a potential disease mechanism.
Interestingly and consistent with the extracellular localization of both enzymes, we showed that the secreted HapA protease from a WT strain was also able to inactivate HlyA that was released by a co-cultured hapA-minus mutant strain.
Both worked well on the recovery of extracellular localization of collagen VI surrounding fibroblasts, while on cell attachment assay, one of them showed remarkable recovery of cell-attachment.
The extracellular localization of AMPs in transgenic plants was confirmed by immunohistochemical methods using sections prepared from leaves and roots of transgenic plants.
The whole-cell biocatalyst system was developed to overcome the cost and complexities associated with enzyme purification via intracellular and extracellular localization of enzymes.
Similarities between apoE and CNTF, including coinciding patterns of postinjury expression, extracellular localization, homologous tertiary structure, and ability to form homodimers led us to examine the possibility that apoE and CNTF directly associate and thereby facilitate the neurotrophic activity of CNTF.
On the other hand, the extracellular localization of enzymes involves the display of enzymes on the surface of the microorganism (thus, the designation "cell-surface display") to avoid possible substrate product transport complexities across the cell membrane (Schüürmann et al. 2014).
Its active site is fully conserved, and the extracellular localization has been determined previously [14].
Hsp90 is also a reported tumor antigen [16], [17], recently explained by its extracellular localization.
