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After protein quantification (Pierce BCA Protein Assay), a total of 5 μg protein was separated on 10% acrylamide/bisacrylamide gels and transferred to nitrocellulose membranes (Amersham Biosciences; Hybond™-C Extra) using a CAPS buffer (10 mM 3-[cyclohexylamino]-1-propane sulfonic acid, pH 11; 10% methanol).
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In other cases, it has to be transmitted as extra information using a maximum of ⌈log2(d)⌉ bits.
2. Add some extra sauce using a small spoon.
With a cheesecloth you can squeeze out extra extract using a compression twist to the peel mixture.
If you want to make the lid extra extra secure, use a hot glue gun to glue down the wire insert or florist wire too.
If you'd like a little extra, use your chopsticks to place a bit of wasabi on top of the nigiri.
The shape, size and materials vary, but every Hanukkah menorah holds nine candles -- one for each day, plus an extra used as the lighter.
If you don't mind spending a little extra, use extra features (bolded text, more pictures, premium placement in searches) to bolster your auctions.
For extra comfort when using a standing desk, a standing mat is a good option.
Some banks have foreign partners that won't levy extra charges for using a foreign A.T.M.
The extra cost of using a meter will add to the family's financial woes.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com