Accordingly, images from a movie, such as those of a dream or a fantasy, can be read at two different levels, an extra-psychical (or objective) one and an intra-psychical one.
Walker says the filmmakers and Al Jazeera English try extra hard to be "objective" in part because of a legacy from the administration ofGeorge W. Bush, which made a concerted effort to brand the channel as a source of dangerous propaganda.
The two extra visits required for objective assessment of PA and accurate evaluation of diet have been omitted in all but two sites (as planned) and dietary assessment reduced to a validated FFQ.
Images (4 central sites/well) were acquired automatically on an IX Ultra confocal platereader (Molecular Devices, Sunnyvale, CA, U.S.A ., equipped with a Plan Fluor 40 × NA0.6 extra long working distance objective and 405 nm/488 nm laser lines for H33342 and sfGFP excitation respectively.
"If I ask for players, or if the club gives me a handful of players, it is like taking a bit the responsibility away from us, and saying we need extra to reach our objectives".
Four central images were obtained per well using a Plan Fluor 40x NA0.6 extra-long working distance objective.
Plates were immediately imaged using an ImageXpress Ultra confocal plate reader, which captured four central images per well using a Plan Fluor 40× NA0.6 extra-long working distance objective.
Fresh HBS was added to each well and the assay plate (four central sites per well) was immediately imaged on the IX ultra confocal plate reader (Molecular Devices, Sunnyvale, CA, USA) using a Plan Fluor 40× NA0.6 extra-long working distance objective.
Images (four central sites per well) were acquired automatically on an IX Ultra confocal plate reader (Molecular Devices, Sunnyvale, CA, USA), equipped with a Plan Fluor 40× NA0.6 extra-long working distance objective and 405/488 nm laser lines for H33342 and cYFP excitation respectively.
