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We designed the extensions to contain alanine residues since alanine lacks an extended side chain that may offer extra interactions with the binding site of the enzyme and complicate this analysis.
Substrate interactions at subsite -7 are provided by residues from loop 145-151 are are thought to create extra interactions with the seventh sugar residue to favor β-CD formation.
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Accessing an extra interaction with the protein via Phe138 gave a significant increase in binding potency compared to similar analogues that do not make this interaction.
I have recently had a spell as captain at Wycombe and the extra interaction with the referees got me thinking about the player-referee relationship.
Remarkably, the extra interaction with IRAK-4 of R70Q led to increased transcript and protein levels of IL-8 without an increase in total NF-κB.
In addition to its canonical D box, yeast Clb5 has an ABBA motif that mediates an extra interaction with Cdc20.
Finally, the flipped base is trapped in an extra-helical location by stabilizing interactions with the protein.
However, a male may actively guard his paternity by engaging in aggressive interactions with the extra-pair male when the latter is trying to copulate with the female (Birkhead and Møller 1992; Møller and Birkhead 1993).
The new modeling results incorporating these extra interactions qualitatively match our experimental findings.
Anisotropic additives show strong interactions with the flow field leading to extra nucleating sites.
Upward going muons and showers produced in neutrino interactions with the surrounding matter will allow the search and study of possible sources of extra-terrestrial neutrinos.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com