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Accessing an extra interaction with the protein via Phe138 gave a significant increase in binding potency compared to similar analogues that do not make this interaction.
I have recently had a spell as captain at Wycombe and the extra interaction with the referees got me thinking about the player-referee relationship.
These latter deviations could be remarkably reduced by introducing the extra interaction energy term, i.e., ΔEex, for the specific counterion binding that was used to correct the observed deviations for the counterion activities.
In addition to its canonical D box, yeast Clb5 has an ABBA motif that mediates an extra interaction with Cdc20.
Remarkably, the extra interaction with IRAK-4 of R70Q led to increased transcript and protein levels of IL-8 without an increase in total NF-κB.
In both cases, MnP4 has an extra interaction in the second coordination sphere of the Ca2+ ions (due to presence of T136 and K296) that could increase its alkaline stability.
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The new modeling results incorporating these extra interactions qualitatively match our experimental findings.
These "extra" interactions foster potent rhomboid inhibition in living cells, thereby opening avenues for rational design of selective rhomboid inhibitors.
2n additional ancillas with appropriate initial states in the computational basis are required to implement the unitary u 0 † (up to arbitrary accuracy) on each register qubit and these extra interactions are shown in the blue dashed box.
The extra interactions between Pro593 in TM6 and Ile528 in TM5 in the inactive conformation may block the rotation and displacement of TM6 needed for LHR activation, making the A593P mutant unable to be activated by LH, as observed experimentally (Table 6).
We designed the extensions to contain alanine residues since alanine lacks an extended side chain that may offer extra interactions with the binding site of the enzyme and complicate this analysis.
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