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The phosphorus removal treatment plant is illustrated via a case study where the addition of extra carbon source and metal salts for improving phosphorus removal are considered.
In contrary results revealed that B. licheniformis WT and recombinant B. licheniformis harboring pHT01-kIspS showed insignificant difference in isoprene production upon utilizing glucose (Fig. 7a) and glycerol (Fig. 7b) as an extra carbon source.
We further examined the cell growth of the WT, deletion mutant, and complemented strains in THY medium with extra carbon source by supplementation with 2% (w/v) gluconate sodium (Fig. 2B).
The highest isoprene production was observed for the recombinant B. subtilis harboring pHT01-kIspS upon utilizing 5 g/L glucose as an extra carbon source, in which it produced 359 µg/L/OD isoprene (Fig. 6a).
The putative chitin synthase (CP4_0468) in NELoc-3 may work in conjunction with the two putative chitinases (CP4_3454 and CP4_3455) in NELoc-1 to take advantage of the chitin as an extra carbon source.
The additional 0.4%% glycerol is also provided in TB broth as an extra carbon source.
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Chlorpyrifos has also been reported to be degraded co-metabolically by bacteria, which needs extra carbon sources (Richins et al. 1997; Mallick et al. 1999).
Moreover, when utilizing extra carbon sources to the media, i.e. glucose and/or glycerol, highest isoprene production was observed for the recombinant B. subtilis at 5 g/L glucose as an extra substrate.
These genes all have ΔAIC values that rank among the top 5% and show bimodality, with one of their two modes containing conditions from the same category, i.e. "adding extra carbon sources".
Nevertheless, one approach is to categorize conditions by the type of perturbations, e.g. heat shock (with different temperature and length of time), hypo-osmotic shock (different time points), and extra carbon sources (different carbon source), etc., and check if one of the two states of a putative switch is enriched within a category of conditions.
She advocates looking for a solution to dealing with "an extra carbon dioxide source" by studying how plants would process the situation.
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