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Figure 2 compares dark-field (i.e. externally illuminated) and bioluminescence (i.e. luminescence emission without external illumination) images of individual V. fischeri cells adhering to the glass window in the presence of 500 nM AI.
Accordingly, no product formation was measured without external illumination at room temperature in our experiments (Supplementary Fig. 5).
It's just infinitely better looking and more responsive than the Kindle — not to mention it has color and doesn't require external illumination.
We have used the conductive AFM to study the local photoresponse of the microcrystalline grains in mixed phase thin films to changes of external illumination.
FRET-sensors have become important tools for intracellular imaging, but their dependence on external illumination presents some limitations, such as photobleaching and phototoxicity, which limit measurements over extended periods of time.
Examples are specially designed camera housings that maintain a constant distance between the camera lens and the sand surface, tripods to fix and maintain the camera angle orthogonal to the sand surface, external illumination systems that guarantee the light level necessary for the image processing algorithms, and special lenses and focusing systems for close proximity image capturing.
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In addition, external light illumination, which in fluorescence-based methods causes phototoxic damage to cells and bleaches the probes by repetitive illumination, is not required for the luminescence reaction; thus, the characteristic properties of the beetle luciferase/luciferin enable longitudinal and quantitative BLI.
c The device shows program/erase endurance by external light illumination of 10 s and negative erase voltage.
In this image most of the background and external light illumination has been removed, and the pupils appear as the brightest part (18).
Efficient bioluminescence resonance energy transfer (BRET) from a bioluminescent protein to a fluorescent protein with high fluorescent quantum yield has been utilized to enhance luminescence intensity, allowing single-cell imaging in near real time without external light illumination.
Hence, good and uniform illumination from external light source is essential for machine vision applications to function.
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