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To compare the extents of uptake between 5 and InsP6, cells were treated in parallel with 5 or InsP6 for 3 h, and the InsPs were then extracted and their concentrations analysed.
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More precisely, the total amount of proteins in the corona seems to impact the extent of uptake.
However, the absolute values were cell-dependent, with COS-7 cells displaying both the highest rate and extent of uptake.
Rates of nucleation and precipitation strongly affect the extent of uptake but the presence of Na+ and K+ has no effect, suggesting formation of solid solution as CaCO3 EuOHCO3.
The pseudo-first-order, pseudo-second-order and intraparticle diffusion kinetic models were used to investigate the adsorption kinetics of fluoride and to quantify the extent of uptake in the adsorption process.
The extent of uptake was highly variable between the metastases, consistent with preclinical rodent studies [8] and clinical data from Morikawa et al. which showed a 60-fold variability of lapatinib uptake (1 to 63 μM) in resected brain metastases of four patients after oral lapatinib [9].
In order to quantitatively validate this difference and to observe the limitations of the CT, we compared the spatial extent of uptake regions from PET and the anatomical correspondences of those regions from MR or CT by manual delineations provided by two expert clinicians (blinded to their drawings).
The kinetics and extent of uptake was quantitated for G-28 and U87 cell lines by flow cytometry (Fig. 1A).
However, in DKOR cells without expression of clathrin, a significant decrease in the extent of uptake of the three states was apparent (Fig. 6B).
In order to quantify the extent of uptake, we made use of the enzymatic activity of the Ure2 protein, which is maintained in its fibrillar state [20], [22].
Using the glutaredoxin activity of Ure2 to determine the extent of uptake, we observed that uptake of all three states of the Ure2 protein was significantly reduced in the absence of clathrin (Fig. 7).
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