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In addition, DUX4-FL expression in myogenic cells is almost exclusive to differentiated myocytes, as identified by expression of myosin heavy chain (MyHC) [ 47]; our previous study reported the number of DUX4-FL-positive nuclei per 1,000 total nuclei in the cultures and thus did not account for possibly differing extents of differentiation among different cultures.
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The extent of differentiation was assessed by combining the more differentiated components (papillary and follicular) together, and comparing these with the less structurally differentiated components (solid and trabecular) combined.
The extent of differentiation was assayed by measuring the activity of glycerophosphate dehydrogenase as well as counting of differentiated versus undifferentiated cells.
Thus, F statistics was used to estimate the extent of differentiation, between groups (populations and sub-populations) (Hartl and Clark 1997).
For those stem cells in contact, the extent of differentiation was fairly linearly related to the extent of contact characterized by coordination number.
We also examined the dynamic time courses of reporter expression representing neuronal differentiation of F11 cells induced by miR-124a and live F11 cells provided in vitro their extent of differentiation efficiency.
Figure 10 shows that a pretreatment of hMSC-TERT cells with GO for 3 days reduced the extent of osteoblastic differentiation, on an average, by 61% in 0.75 mM GO-treated cells and by 97% in 1 mM GO-treated cells, as compared with the extent of differentiation in untreated controls.
Because changes in the cellular membrane and cytosol during differentiation were shown to influence membrane adsorption and cellular internalization, we could successfully evaluate the extent of differentiation simply like stain indicators.
Marker of pluripotency, Oct-4 was used to document the extent of differentiation.
In vitro treatment with AMD3100 did not increase apoptosis nor did it alter the extent of differentiation (Figure S4).
It is of interest to note that different differentiation markers did not give identical estimates of the extent of differentiation in our samples.
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