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We find that the extent of transcript reduction represents a continuum of susceptibilities of transcripts to virus-mediated shutoff.
Here we present a detailed, transcriptome-wide analysis of host shutoff, with careful microarray normalization to allow rigorous determination of the magnitude and extent of transcript loss.
Importantly, Hoxa5 and Hoxb5 play a role in morphogenesis and early differentiation of lung [123], [124] and the extent of transcript expression correlates with the level of methylation [125].
The nature and extent of transcript variation differs across tissues.
Convergent gene pairs were ranked by the extent of transcript overlap (Brar et al., 2012a ).
The extent of transcript KD, as determined by Q-PCR, correlated once more with significant loss of clonogenic potential.
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Once the extent of transcripts was determined using this combinatorial approach, complete sequences were amplified by PCR and Sanger sequenced resulting in complete sequence of the coding regions and the ability to predict the underlying polypeptide.
With the identification of UIF, the repertoire of mRNA export adaptors is expanded and now provides a clear framework to explore the extent of transcript-specific mRNA export in human cells.
Figure 1A depicts the ATG31 – SES1 locus, showing the location of the tV UAC D tDNA, and the extent of transcripts normally produced from the region.
Before the RNA-Seq coverage data were introduced, polyadenylated cDNAs were used to set the 3′ extent of transcripts for FlyBase gene models.
As a consequence the expressional level of a transcript depends on the extent of transcriptional regulation and the proportion of cells expressing the transcript.
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