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The extent of the linker between the targeting device and the epigenetic modifier is the main determinant of the possible reach distance.
It is notable that the extent of the linker motion is not exclusively dependent on the number of atoms in the linker connecting the glycan to the surface (i.e., the linker backbone atoms).
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The curing conditions affected the extent of the cross-linker hydrolysis.
A decrease in the extent of cross-linker hydrolysis by 50 80% was achieved in all cases as the temperature of the curing was increased, in stages, from 160 °C to 200 °C.
3) The use of an additional (protein) cross-linker raises concern as to the extent of the "directness" of specificity of physical interactions by which DKC1 impacts transcription in cells.
Furthermore, the structure of the linker has more influence on labeling yield and stability than the linker length.
Secondly, because of the dimerization, the movement of the linker peptides can be translated into a relative movement between the linker peptide and the β-hairpin in each subunit, thus releasing of the β-hairpin from the linker peptide.
Furthermore, the amide function increases the rigidity of the linker.
Residues of the linker are numbered as 101 105.
Substitution of the linker region (GS-Sub) weakens the binding with Ub considerably, whereas both insertion (GS-Ins) and substitution of the linker region with RAP80 linker (RAP80-Linker) significantly increase its binding affinity with Ub (Table 1).
The observed rates and extents of hydrolysis were lower for the CD than for the intact Cel7A, suggesting the specific activity (i.e. turnover rate) of the bound enzyme was significantly reduced by the absence of the linker and CBM.
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