This affects the distribution of the extent of staining as compared to studies that have excluded tumour stroma and calculated the proportion of stained cells in the tumour parenchyma only.
In addition to the extent of staining, tissue and cellular distribution of staining was determined.
Compared with untreated GS animals, intensity and extent of staining for renal α-SMA, TGF-β1, Col IV, and FN were markedly reduced in glomerulus, mesangial cells, and tubular interstitium of GS rats treated with either ATRA or benazepril.
However, the extent of staining varied among the groups.
The evaluation was based on the staining intensity and extent of staining.
The staining intensity and the extent of staining were determined for each organ using both antibodies.
The product of the intensity and the extent of staining yielded final scores ranging between 0 and 16.
The extent of staining ranged from the majority of tumour cells to scattered positive cells in some sections.
The extent of staining ranged from negative to moderate with few high-grade tumours demonstrating no epithelial or stromal staining.
Thus, the score ranged from 0 to 6 and included the average intensity and extent of staining.
For β-catenin staining evaluation, any intensity and extent of staining found in the nucleus was considered positive.
