Not only having a larger number of dehydration-responsive genes, but the extent of the responsiveness of GmHKs, i.e. the fold change, was also higher in shoot tissues.
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Further work with directly comparable datasets is needed in order to better understand the extent of conservation of nourishment-responsive gene expression across taxa.
Fig. 2A illustrates that as little as 25 ng/ml IL-24 led to a robust activation of STAT3 and to a much lesser extent of STAT1 and 5 in responsive HaCaT cells.
However, the technique is not quantitative and therefore a clinically useful relationship between fold-induction of selected hypoxia-responsive genes and extent of hypoxia cannot be defined.
Second, and as expected, the level of iron present in the growth medium affects the extent of H2O2 regulation; some genes were more responsive than others, likely through the modulation of PerR activity.
Fig. 5 Validation of responsive unannotated transcripts.
(D4 ) Comparison of responsive and non-responsive GoCs firing rates.
After 24 hours of incubation, transfected synovial fibroblasts remained responsive to IL-1β, although the extent of IL-6 induction was slightly lower than in non-transfected cells.
