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When expression data for each replicate were plotted against each other, RPKM values were observed to be adequately reproducible, though expectedly, the extent of reproducibility was slightly less in genes with low expression [ 17].
When conducting pharmacoepidemiologic studies, it has been recommended that at least two cohorts be evaluated to establish the extent of reproducibility [ 33].
When conducting pharmacoepidemiological studies, it has been recommended that at least two cohorts be evaluated to establish the extent of reproducibility.
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Leaders of the project say it will ultimately benefit the field by gauging the extent of the reproducibility problem in cancer biology.
A measure of the extent of the reproducibility of the sugar profiles obtained from the pigmented systems with respect to the unpigmented ones can be expressed by calculating the correlation coefficients.
These explicit recommendations are most likely to improve pathological assessment of disease extent and reproducibility of grade of DCIS and may improve detection of invasive disease.
The use of an internal reference overcomes to some extent the lack of reproducibility which is well known to be the main limitation of this type of postcontrast perfusion data.
Due to the absence of similar studies in other tumour entities, e.g. lung cancer, it remains unclear to what extent the reproducibility of FMISO will be affected by the lack of rigid immobilization and the presence of respiratory motion.
In the future, the challenge will be to develop these methods further to combine statistical assessment of distributions with measures of reproducibility and extent in the analysis of digital gold signal.
However, accuracy of defining the extent of cartilage lesions and reproducibility in grading of lesions are not optimal.
Benefits of a recombinant expression system include control over the expressed protein sequence, control over extent of posttranslational modifications, and reproducibility of culturing conditions and hence protein composition [ 11– 16].
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