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Protein size, expression level, distribution between soluble and insoluble forms, and extent of purification were monitored by SDS-PAGE.
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After reversal of the cross-linking and DNA purification, the extent of enrichment was monitored by PCR amplification.
While many research pursuits begin with protein purification, the extent of material required is a burden somewhat unique to protein crystallography.
Studies have not yet validated this method extensively, particularly with regard to the stability of individual folate forms and the extent of their interconversion during the purification and separation procedures.
After reversal of the crosslinks and DNA purification, the extent of enrichment was monitored by PCR amplification of promoters using forward and reverse primers to the hTR (5'-TACGCCCTTCTCAGTTAGGGTTAG-3' and 5'-AGCCCGCCCGAGAGAGTGAC-3') gene promoter fragments [Zhao, 2003 #9] and to the GAPDH coding region as a negative control (5'-TGAAGGTCGGAGTCAACGGATTTGGT-3' and 5'-CATGTGGGCCATGAGGTCCACCAC-3').
Rite of purification?
It was a necessary process of purification.
"This will be an act of purification.
In 1744, he launched a campaign of purification and renewal.
This is a time of purification for the church.
"Abraham's act of purification is celebrated today".
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