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By controlling the reaction time or the molar ratio between the reactants, this synthetic strategy makes it possible to tune the extent of modification of PVA for a given molecular weight of grafts.
Incubation with PAO markedly reduced the extent of modification of H-Ras by 15d-PGJ2-B in COS-7 cells (Fig. 4D).
However, the link between stocking intensity and the extent of modification of local genetic diversity, including allelic richness and admixture, have been much less investigated (Hansen 2002; Marie et al. 2010; Perrier et al. 2011, 2013b; Lamaze et al. 2012).
The pyrrole modification may be spread over the α- and β-chains of haemoglobin and it is possible that the extent of modification of haemoglobin is too low to be observed in the predominant unmodified haemoglobin pool.
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It was found that pore volume had the greatest influence on the extent of modification.
However, subsequent experiments indicated that the extent of modification is much greater for smaller beads.
Our experimental setup was designed for identification of differentially reactive sites rather than for quantification of small changes in extent of modification.
Since reactions that form AGE are not reversible, AGE slowly accumulate over the lifetime of a protein, and the extent of modification is thus a function of protein turnover.
The RNAs mapped in this study were each subjected to a titration of these reagents in order to optimize the extent of modification and the corresponding efficiency of reverse transcription.
The extent of modification was determined by measuring thiobarbituric acid-reactive substances (TBARS).
To determine if such alterations are a general property of the repressor, we assessed the extent of coordination of modifications on all individual blocks of affected chromatin.
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