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Significantly, three tendencies for the folded/unfolded equilibrium are observed: increasing solubility and polarity of the medium and decreasing temperature results in a higher extent of folding.
Initial analyses provide a judgment of sample quality, revealing the potential presence of aggregation, the overall extent of folding or disorder, the radius of gyration, maximum particle dimensions and oligomerization state.
Based on these results, STM promoter activity can thus be considered as a read-out of the extent of folding in the boundary of the SAM.
Nonetheless, the unassembled swine HEV protein was recognized both efficiently and specifically by a reference swine serum raised against the naïve virus [ 25], and was highly immunogenic in mice, suggesting some extent of folding and conservation of its antigenic structure.
Prefrontal cortical folding (gyrification) was measured bilaterally by an automated version of the automated-gyrification index (A-GI), a ratio reflecting the extent of folding, from the slice containing the inner genu of the corpus callosum up to the frontal pole.
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The extents of folding in these experiments compared to those obtained at equilibrium indicates a reaction time of 9 ± 2 μs, which is an upper limit to the droplet lifetime because some folding will occur in the Taylor cone prior to droplet formation.
In addition, the slopes of the linear regression fitting curves indicated that the Applied Biosystems data set has overall less ratio compression of the extent of fold change (fitted slope 0.63 0.72) compared to the Agilent array data set (fitted slope 0.19 0.23).
These observations were also confirmed in ARO cells, even though the extent of fold activation was lesser than in HEK293 cells because of a lower transfection efficiency (data not shown).
Although the extent of fold-change (FC) varied between the qRT-PCR and microarray results (Table 3 and Figure 2), the differential expression patterns were coincident, indicating the reliability of the microarray analysis.
To find the genes with the most robust changes in expression, the data was plotted as a "Volcano Plot" (Supplementary Figure S2B), which allows statistical significance to be measured along with the extent of fold change in expression.
Furthermore, the extent of intramolecular folding of the MET16 monomer correlates well with cosolute size.
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