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This assay is used to determine the extent of cell viability in cultures after various treatments.
Moreover, the pattern of Lon expression was able to reflect on the extent of cell viability under different periods of exposure of hypoxia.
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Interestingly, in the Fas-resistant SKRC-07 cells, the downregulation of Bcl-2 did not induce a loss of cell viability to the extent seen in the sensitive line treated with IFN α and anti-Fas.
Alamar blue both fluoresces and changes color in response to chemical reduction, and the extent of the conversion is a reflection of cell viability (product literature, Trek Diagnostic Systems).
The extent of reduction of MTT to formazan within cells was quantified spectrophotometrically at 595 nm and taken as an indicator of cell viability [45].
The results of cell viability are shown in Fig. 7.
Figure 5 Direct contact measurements and calculations of cell viability.
Hence 77% of cell viability could be observed (Fig. 3b).
IC50 value (concentration of compounds inhibited 50% of cell viability) was determined from the graph of cell viability vs absorbance.
Cell viability data were expressed as percentage of cell viability calculated.
Determination of cell viability.
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