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MDA content serves as an indicator of the extent of lipid peroxidation and is an indirect reflection of the extent of cell damage (Wang et al. 2011b).
The extent of cell damage was evaluated in terms of lactate dehydrogenase leakage from the cells and altered intracellular redox status.
The level of MDA reflects the extent of cell damage by oxidative stress [ 33].
Meanwhile, to evaluate the extent of cell damage caused by cold stress in CaMBF1-expressing seedlings, electrolyte leakage was measured.
Once the cells are damaged, LDH was then released into extracellular, the extent of cell damage can be detected by LDH activity [ 40].
These results suggest that the E. coli membrane composition is relevant regarding the extent of cell damage caused by oxidative stress elicitors.
Similar(49)
Although miR-133 is abundantly expressed in skeletal muscles, unlike in AMI, acute hind-limb ischemia did not result in increased plasma levels of the miRNAs (miR-1 and miR-133), suggesting that the release of miRNA into the systemic circulation may possibly be tissue or miRNA specific and dependent on the extent and the timing of cell damage [ 48].
Treatment with melatonin significantly attenuated the increased level of serum aminotransferase, reduced the severe extent of hepatic cell damage, steatosis and the immigration of inflammatory cells, but had no effects on hepatic expression of lipogenic genes.
Epithelial injury was assessed by measuring lactate dehydrogenase (LDH), an intracellular enzyme that when present in the medium reflects the extent of epithelial cell damage and lysis.
The release of lactate dehydrogenase (LDH) from epithelial cells into the cell-culture medium was measured to quantify the extent of epithelial cell damage.
In conclusion, the extent of intestinal cell damage is related to shock, ISS, and abdominal trauma.
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