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An index is proposed to measure the repetitive extent of batch processes.
A previous attempt to assess the extent of batch effects and the effectiveness of batch adjustment methods was made by the MAQC-II project [29].
Unsupervised clustering was performed to assess the extent of batch effects.
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The upper bars of Figure 5 show the extent of impact for mass spectrometers and for nested batch on each of the metrics individually, for finer resolution.
The effect has also been shown to be correlated with differences in batch sizes and the extent of homogeneity of samples in each batch.
Without such information, all correlations between the measured conductivity and the major processing parameters (viscosity, extent of reaction) remain strictly batch-specific and empirical.
(48) Our lab, as well as other laboratories, previously reported using gel electrophoresis to determine the extent of conjugation of a given batch of QD conjugates.
As expected, after implementation of intra-platform batch adjustment, the samples were intermingled on the basis of the biological variable (Fig. 2b); this situation means removal of dataset-specific biases and elimination of batch effects to a greater extent (though not complete elimination) and may facilitate clinically important analysis of the underlying biology.
In one of three batches of 5-day-old A↓ cells, the dominating HS FeII doublet was still observed by MB (Supporting Information Figure S1), indicating some batch-to-batch variation in the extent of reoxidation.
This would introduce a high extent of variability due to mixing different batches of embryos (in our hands the number of chimeric embryos is about ten for each experiment), that might hinder the interpretation of the results.
The extent of damage of cells during dead-end batch centrifugation is determined primarily by a combination of the extent of exposure to centrifugal forces (product of RCF and spin time) and the method of cell pellet resuspension.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com