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We reasoned this would require exceptionally high selection stringency given the extent of background phenotypic variation and the presumed genetic uniformity of the clone.
In order to investigate the extent of background phosphorylation of HSL purified from Sf9 insect cells, aliquots of preparations of purified HSL isoforms were incubated with calf intestinal phosphatase before assaying triglyceride lipase activity.
The choice of performing in parallel FC and HAI assay was motivated by the transient expression of the fixing complement antibodies, which renders the test more useful in studies of recent infections [20] [21], and may partially correct the seroprevalence values estimated on the basis of HAI, that shows a variable extent of background positivity [7].
Our assessment of the total B19 mRNA signal (Fig. 3C) took into account both the amount of DNA amplified by PCR (Fig. 3B) in absolute numbers and the extent of background DNA signal obtained by RT-PCR in the absence of reverse transcriptase.
The DFE affects both the extent of background selection and hitch-hiking.
The extent of background selection, hitch-hiking and clonal interference can be evaluated using the diagnostic statistics presented here.
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Hence, besides the spatial extent of buildings, background information of a building always includes four attributes: number of rooms, number of storeys, structure type, and construction cost.
To assess the extent of assay background resulting from non-specific antibody binding, replicate samples (adjacent sections) were incubated with the VeraTag labeled anti-HGF antibody and an isotype (IgG DA1E) in place of the anti-MET antibody.
Therefore the ChIP-seq signal in unstimulated HeLa cells consists to a large extent of unspecific background sequences [3]. Figure 3 suggests furthermore a PWM score of 30 as threshold for binding sites exhibiting a marked difference in occupation from background levels.
To gauge the maximal extent of this background, we examined mRNAs that are expressed specifically in somatic tissues.
Although additional studies, with a variety of platforms, are needed to carefully dissect the machine noise component from the mutational flicker, it is clear that the extent of such background variation is greater than was previously thought.
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