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Our study exemplifies that the extent of alternative splicing is presumably much higher than previously estimated and that alternatively spliced transcripts presumably can generate molecules of deviating function compared to their constitutive counterpart.
Given the extent of alternative splicing and array manufacturing variation, however, a single probe can be misleading.
The extent of alternative splicing, as well as the balance between types of alternative splicing (e.g. alternate 5'SS versus exon-skipping splicing), differs by organism [15].
We initiated an analysis of the extent of alternative mRNA editing by large scale sequencing of full-length cDNAs from the bloodstream developmental stage of T. brucei [11], [15].
The extent of alternative splicing of honeybee neurexin I transcripts was investigated by conducting two series of RT-PCR reactions from honeybee cDNA, using primers corresponding to the predicted start and alternate stop codon regions of AmNrxI-A and AmNrxI-B.
Additionally, the nature and extent of alternative splicing in Toxoplasma gondii is unknown.
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In particular there were differing expectations about the nature and extent of alternatives that might be considered.
We tested the extent of valid alternative reading frames among this large set of TR-proteins.
This is also supported by the extent of NAGNAG alternative splicing based on NGS data which is in the same range as in flowering plants [ 65].
Such a targeted alignment strategy has been highly effective in studying the extent of intragenic alternative splicing in the human genome, even in short reads of only 32 nt [ 20- 23].
Using the single copy Puroindoline a and b genes (Pina and Pinb) we have characterised the extent of these alternative and antisense transcripts and based upon these results we speculate that such sequences might play a role in grain development.
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