He had a formula: Prepare extensively every time, be funny ("Every seven to nine minutes I'll have them laughing"), and frequently reinforce the broader message ("I make certain that every five minutes I'm giving them a concept, an idea, a process, a hope builder").
While Kriegel wasn't a co-founder at OMGPOP, he was with the startup through the extensively difficult time before the company found a hit on the iOS platform in Draw Something.
And I do often give in, but if I am such a nice sister, I think I deserve more praise from you! Natalie: You must admit that I thank you extensively every time you buy me things.
While this is so, the functional complement of the genome changes extensively across time and between lineages; therefore, evidence of the current action of purifying selection in humans is essential.
The histone-antibody complexes were washed extensively nine times with washing buffers containing a different concentration of salt (first washing buffer, three times with 75 mM NaCl; second washing buffer, three times with 125 mM NaCl; third washing buffer, three times with 175 mM NaCl in Tris-HCl pH 7.5 and 10 mM EDTA).
Cells were washed twice with HBSS and blocked with HBSS containing 4% BSA for 15 min. Cells were then incubated at 37 °C with biotin α-Flag antibody (1 μg/mL, Sigma) for 30 min, washed five times followed by an incubation with streptavidin-conjugated Qdot-655 (0.1 nM, Invitrogen) for 2 min, washed extensively eight times, and immediately mounted on an Olympus inverted microscope (IX51).
He had very little to tell them since he's now been jailed twice for his protest activity, and questioned extensively both times.
The ARCH model as developed by Engle (1982) is an extensively used time-series models in the finance-related research.
The erythrocyte ghosts were collected at the top of the free parasite pellet, washed extensively five times in the hypotonic buffer.
