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Tumors were dissected, weighted and fixed overnight in 4% paraformaldehyde at 4°C, washed extensively in PBS, saturated with increasing concentrations of sucrose and embedded in O.C.T. (Tissue-Tek) for preservation at -80°C or in paraffin.
During biohydrogenation, unsaturated fatty acids (linoleic and alpha-linolenic acids) are extensively hydrogenated to form saturated fatty acids (stearic acid 18 0 and palmitic acid 16:0).
Lindane is a chlorinated cyclic saturated hydrocarbon used extensively for the control of agricultural pests and mosquitoes.
The hybridoma supernatant was clarified by centrifugation and proteins concentrated by precipitation with 45% saturated ammonium sulphate, dialysed extensively against PBS and antibodies purified on protein G chromatography (HiTrap protein G column, GE Lifesciences; [58]).
Saltern crystallizers (NaCl saturated) have been studied extensively by molecular methods and prove to be among the simplest known communities in terms of species composition by both classical microbiological and molecular methods [ 7- 12].
The resulting organic phase consisted of aliphatic hydrocarbons (paraffins and olefins), aromatic hydrocarbons (extensively alkylated non-oxygenated mono-aromatics, mainly alkylbenzenes as well as mono-aromatic oxygenates, mainly phenolics), condensation products (mainly naphthalenes) and saturated oxygenates (ketones and carboxylic acids).
Everywhere saturated.
The market is saturated.
The market appeared saturated.
I was just saturated.
The rain saturated Bolton.
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